Format

Send to

Choose Destination
Br J Cancer. 1998 Jun;77(12):2349-56.

Moderate amplifications of the c-myc gene correlate with molecular and clinicopathological parameters in colorectal cancer.

Author information

1
Institut de Recerca Oncològica (Department of Cancer and Metastasis), Hospital Duran i Reynals, Barcelona, Spain.

Abstract

C-myc gene activation is a common event in multiple types of neoplasia and has been associated with different cellular processes relevant to the malignant transformation of cancer cells. C-myc gene amplification has been analysed in colorectal carcinomas by means of an innovative DNA fingerprinting method based on the arbitrarily primed PCR. This method requires a low amount of DNA, uses multiple internal controls and appears sensitive and reproducible. Clinicopathological and molecular correlates have been investigated in a series of 70 colorectal carcinomas. The incidence of c-myc amplification was 26%, ranging from two- to fivefold increase in copy number. C-myc amplification occurrence was more frequent in more advanced stages of tumour invasion (P < 0.001) and was associated with mutations in the p53 tumour-suppressor gene (P = 0.048). The presence of c-myc amplification was indicative of a shorter disease-free survival period but, because of its strong association with Dukes' stage, its prognostic value is questionable.

PMID:
9649157
PMCID:
PMC2150408
DOI:
10.1038/bjc.1998.390
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center