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Cell Transplant. 1998 May-Jun;7(3):309-17.

Single-cell suspensions of cultured human keratinocytes in fibrin-glue reconstitute the epidermis.

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Department of Plastic and Hand-Surgery, Medical School, Albert-Ludwigs-University, Freiburg i.Br., Germany.


To overcome common disadvantages of standard cultured epidermal sheet grafts (CEG) we have developed a new technique of transplanting cultured human keratinocytes suspended as single cells in a fibrin-glue matrix (Keratinocyte-fibrin-glue suspension-KFGS). In an athymic mouse model with reproducible standardized full thickness wounds this new technique was compared directly to CEG. Reepithelialization was similar in both groups, but reconstitution of the dermo-epidermal junction zone, as shown by electron microscopy and immunohistochemistry was significantly enhanced by the fibrin-glue suspension technique. The new KFGS technique is earlier available than sheet grafts, is able to transfer actively proliferative single keratinocytes, and simplifies the application.

[Indexed for MEDLINE]

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