Format

Send to

Choose Destination
See comment in PubMed Commons below
J Mol Biol. 1998 Jun 26;279(5):1027-43.

DNA microloops and microdomains: a general mechanism for transcription activation by torsional transmission.

Author information

1
MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England.

Abstract

Prokaryotic transcriptional activation often involves the formation of DNA microloops upstream of the polymerase binding site. There is substantial evidence that these microloops function to bring activator and polymerase into close spatial proximity. However additional functions are suggested by the ability of certain activators, of which FIS is the best characterised example, to facilitate polymerase binding, promoter opening and polymerase escape. We review here the evidence for the concept that the topology of the microloop formed by such activators is tightly coupled to the structural transitions in DNA mediated by RNA polymerase. In this process, which we term torsional transmission, a major function of the activator is to act as a local topological homeostat. We argue that the same mechanism may also be employed in site-specific DNA inversion.

PMID:
9642081
DOI:
10.1006/jmbi.1998.1834
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center