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J Chromatogr A. 1998 May 8;806(1):169-85.

Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.

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1
School of Biology, Georgia Institute of Technology, Atlanta 30332, USA.

Abstract

Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.

PMID:
9639888
[Indexed for MEDLINE]

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