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Biotechnology (N Y). 1995 Nov;13(11):1225-9.

Expression of the human muscarinic receptor gene m2 in Dictyostelium discoideum.

Author information

1
Institut für Pharmazeutische Biologie, Johann Wolfgang Goethe Universität, Frankfurt, Germany.

Abstract

We have expressed a functional human muscarinic M2 receptor, under the control of the homologous discoidin I gamma promoter, in the cellular slime mold Dictyostelium discoideum. The use of a contact site A leader peptide ensured insertion of the newly synthesized receptor protein into the plasma membrane. Due to the characteristics of the discoidin I gamma promoter, the M2 receptor is expressed during late growth and early development. The heterologously expressed M2 receptors show binding characteristics similar to authentic receptors. Membranes as well as whole cells can be used in ligand binding assays.

PMID:
9636297
DOI:
10.1038/nbt1195-1225
[Indexed for MEDLINE]

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