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J Appl Microbiol. 1998 Feb;84(2):176-84.

Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence.

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Centre de recherche en sciences et technologie du lait (STELA), Université Laval, Ste-Foy, Québec, Canada.


A highly specific antisera was produced in New Zealand white rabbits against nisin Z, a 3400 Da bacteriocin produced by Lactococcus lactis ssp. lactis biovar. diacetylactis UL 719. A dot immunoblot assay was then developed to detect nisin Z in milk and whey. As few as 1.5 10(-1) international units per ml (IU ml-1), corresponding to 0.003 microgram ml-1 of pure nisin Z, were detected in carbonate-bicarbonate buffer within 6 h using chemiluminescence. When milk and whey samples were tested, approximately 0.155 microgram ml-1 (7.9 IU ml-1) of nisin Z was detected. The detection limit obtained was lower than that of traditional methods including microtitration and agar diffusion.

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