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Cytokine. 1998 Jun;10(6):418-22.

Interferon gamma gene expression in rat central nervous system glial cells.

Author information

1
Laboratory of Organ and System Pathophysiology, Istituto Superiore di Sanità, Rome, Italy.

Abstract

The expression of interferon gamma (TFN-gamma) mRNA in purified cultures of glial cells and leptomeningeal fibroblasts from the neonatal rat brain was analysed using semi-quantitative reverse transcriptase-polymerase chain reaction. IFN-gamma specific transcripts were clearly detected in rat astrocytes and were upregulated after treatment with IFN-gamma itself or cycloheximide. In microglial cells, IFN-gamma transcripts were barely detectable in basal conditions and were upregulated by lipopolysaccharide and, to a lesser extent, by IFN-gamma or cycloheximide. IFN-gamma mRNA was neither detected not inducible in oligodendrocytes and leptomeningeal cells. The demonstration that IFN-gamma is expressed in astrocytes and microglia widens the putative role of these cells in local immunoregulation and antiviral responses.

PMID:
9632527
DOI:
10.1006/cyto.1997.0314
[Indexed for MEDLINE]

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