Format

Send to

Choose Destination
J Virol Methods. 1998 Mar;71(1):1-6.

Simple one-tube reverse transcription-polymerase chain reaction protocol containing anticontamination procedure for detection of GB virus C/hepatitis G virus RNA.

Author information

1
Institute of Microbiology and Immunology, Medical Faculty of Ljubljana, Slovenia. poljak@ibmi.mf.uni-lj.si

Abstract

A simple one-tube reverse transcription-polymerase chain reaction protocol for detection of GB virus C/hepatitis G virus (GBV-C/HGV) RNA in serum samples containing anticontamination uracil-N-glycosylase procedure was developed. Amplification products were detected in a standard microtiter plate format using a commercial PCR ELISA kit (Boehringer Mannheim, Germany). Complete agreement between the results of a novel method and the previously determined GBV-C/HGV RNA status was obtained on 131 serum samples (76 GBV-C/HGV RNA positive and 55 GBV-C/HGV RNA negative samples). The novel method provides the potential for automated, simple, rapid and accurate routine detection of GBV-C/HGV RNA in diagnostic virological laboratories.

PMID:
9628215
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center