Format

Send to

Choose Destination
Biochem Biophys Res Commun. 1998 May 29;246(3):788-91.

Regulation of cellular adhesion to extracellular matrix proteins by galectin-3.

Author information

1
Department of Biochemistry, Meharry Medical College, Nashville, Tennessee 37208, USA. ochien10@ccvax.mmc.edu

Abstract

The control of cellular adhesion to extracellular matrix proteins is poorly understood. In the present analyses, we set out to test the hypothesis that high galectin-3 concentration on the cell surface downregulates cellular adhesion to the extracellular matrix proteins. Various tumor cell lines were briefly incubated without or with galectin-3 and then allowed to adhere to wells coated with laminin-1, collagen IV and fibronectin. Our data demonstrated that the cells which were incubated with galectin-3 prior to plating had significantly reduced adhesion to extracellular matrix proteins. This inhibition involved the carbohydrate recognition domain of the lectin because adhesion was achieved in the presence of galectin-3 and lactose but not galectin-3 and sucrose. Furthermore we demonstrated that galectin-3 associates with alpha 1 beta 1 integrin in a lactose dependent manner.

PMID:
9618290
DOI:
10.1006/bbrc.1998.8708
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center