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Methods. 1998 Apr;14(4):393-406.

Lineage analysis using retroviral vectors.

Author information

1
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

Knowledge of the genealogical relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Genealogical relationships can be revealed by a variety of methods, all of which involve marking a progenitor cell and/or a group of cells and then following the progeny. The use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues is described. An overview of the relevant aspects of the retroviral life cycle is given, and the strategies and current methods in use in our laboratory are described.

PMID:
9608510
DOI:
10.1006/meth.1998.0594
[Indexed for MEDLINE]

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