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Methods. 1998 Apr;14(4):355-65.

Use of FLP/FRT system to study Drosophila development.

Author information

1
Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.

Abstract

Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both developing tissues and adult cuticle. Strains carrying these genetically marked FLP/FRT chromosomes have greatly enhanced our ability to study gene function in both germline and somatic Drosophila cells.

PMID:
9608507
DOI:
10.1006/meth.1998.0591
[Indexed for MEDLINE]

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