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Changgeng Yi Xue Za Zhi. 1998 Mar;21(1):63-6.

A quick method to extract DNA from paraffin-embedded tissues.

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Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C.



The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA.


We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method.


The amplified products from our method were superior to that of the conventional method.


The method we propose has a better recovery of DNA and is more time efficient.

[Indexed for MEDLINE]

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