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Changgeng Yi Xue Za Zhi. 1998 Mar;21(1):63-6.

A quick method to extract DNA from paraffin-embedded tissues.

Author information

1
Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C.

Abstract

BACKGROUND:

The amplification power of the polymerase chain reaction (PCR) technique has had great impact in molecular analysis, and DNA extraction is a common requirement in retrospective studies utilizing PCR as a tool. Conventional methods used in deparaffinization, harvesting and purification of DNA from paraffin-embedded tissue are time consuming and cause a significant loss in the yield of DNA.

METHODS:

We utilized heating-melting-cooling removal of paraffin, digestion of the sample with proteinase K and purification of the extracted DNA by a microconcentrator. The products, after PCR amplification of the p53 gene exon 8, were used to make a comparison between our method and the conventional xylene-phenol-choloform method.

RESULTS:

The amplified products from our method were superior to that of the conventional method.

CONCLUSION:

The method we propose has a better recovery of DNA and is more time efficient.

PMID:
9607266
[Indexed for MEDLINE]

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