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J Am Vet Med Assoc. 1998 May 15;212(10):1569-71.

Comparison of serum cortisol concentrations in clinically normal dogs after administration of freshly reconstituted versus reconstituted and stored frozen cosyntropin.

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  • 1Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.

Abstract

OBJECTIVE:

To evaluate the biological stability of reconstituted cosyntropin after storage at -20 C for 2, 4, and 6 months.

DESIGN:

Prospective study.

ANIMALS:

10 clinically normal dogs.

PROCEDURE:

Serum cortisol concentrations in dogs were determined before and 1 hour after administration of freshly reconstituted cosyntropin (synthetic ACTH) or cosyntropin that had been reconstituted and stored frozen at -20 C in plastic syringes for 2, 4, and 6 months. Cosyntropin was administered at 5 micrograms/kg (2.3 micrograms/lb) of body weight, i.v.

RESULTS:

Baseline serum cortisol concentrations were similar at each sampling time. Compared with the effects of freshly reconstituted cosyntropin, administration of cosyntropin that had been frozen did not have a significantly different effect on serum cortisol concentrations.

CLINICAL IMPLICATIONS:

Cosyntropin can be reconstituted and stored frozen at -20 C in plastic syringes for 6 months with no adverse effects on bioactivity of the polypeptide.

PMID:
9604025
[PubMed - indexed for MEDLINE]
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