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Appl Environ Microbiol. 1998 Jun;64(6):2240-6.

New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.

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  • 1Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby, Denmark.


Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.

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