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FEBS Lett. 1998 Apr 10;426(1):62-6.

Maize polyamine oxidase: primary structure from protein and cDNA sequencing.

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1
Dipartimento di Biologia, Università Roma Tre, Rome, Italy.

Abstract

The first complete amino acid sequence of a flavin-containing polyamine oxidase was solved by a combined approach of nucleotide and peptide sequence analysis. A cDNA of 1737 bp, isolated from maize seedlings by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends strategies, was cloned and its sequence determined. This cDNA contains information for a polypeptide chain of 500 amino acids. Its amino-terminal sequence shows the typical features of secretion signal peptides. The primary structure of the mature protein was independently confirmed by extensive amino acid sequencing. Structural relationships with flavin-containing monoamine oxidases are also discussed.

PMID:
9598979
[Indexed for MEDLINE]
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