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Nat Biotechnol. 1998 May;16(5):431-9.

Construction of YAC-based mammalian artificial chromosomes.

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1
Department of Molecular Biology, School of Science, Nagoya University, Japan.

Abstract

To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.

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PMID:
9592390
DOI:
10.1038/nbt0598-431
[Indexed for MEDLINE]

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