Format

Send to

Choose Destination
Nucleic Acids Res. 1998 May 15;26(10):2508-10.

New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.

Author information

1
GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology and Tumour Genetics, Marchioninistrasse 25, 81377 Munich, Germany. karreman@gsf.de

Abstract

Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.

PMID:
9580709
PMCID:
PMC147567
DOI:
10.1093/nar/26.10.2508
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center