Format

Send to

Choose Destination

Prednisolone inhibits synthesis of 5-H(P)ETE in eosinophils from asthmatic patients during a wheezing attack but not during remission.

Author information

1
First Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama, Japan.

Abstract

To estimate the effect of prednisolone on 5-lipoxygenase activity in eosinophils obtained from asthmatic patients, cytosolic levels of 5-H(P)ETE and Ca2+ were measured in the eosinophils which were exposed to prednisolone in vitro and in vivo. The mean level of 5-H(P)ETE during a wheezing attack was significantly lower in the patients who had received intravenous prednisolone (500 mg/day). Incubation with prednisolone in vitro caused a dose-dependent decrease in the cytosolic levels of 5-H(P)ETE and Ca2+ in eosinophils obtained during the wheezing attack, but not in the eosinophils obtained from during remission. Results suggest that prednisolone inhibits the level of 5-H(P)ETE in the eosinophil cytosols of asthmatic patients during a wheezing attack, probably by inhibition of 5-lipoxygenase activity which is involved in the reduction of the influx of Ca2+.

PMID:
9578148
DOI:
10.1016/s0952-3278(98)90149-1
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center