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Biochem Biophys Res Commun. 1998 Apr 17;245(2):459-65.

Cytochrome c oxidase does not catalyze the anaerobic reduction of NO.

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Department of Biochemical Sciences, University of Rome La Sapienza, Italy.


A possible role of reduced cytochrome c oxidase in the metabolism of nitric oxide (NO) has been examined with amperometric and stopped-flow photometric techniques. Reduced purified cytochrome c oxidase and mitochondria showed no catalytic reaction with NO under anaerobic conditions within more than 30 minutes. Only fast binding of NO to the reduced enzyme in a 1:1 stoichiometric ratio was observed. The NO binding rate was strongly decreased in the presence of 1 mM cyanide. These data indicate that, contrary to previous proposals, cytochrome c oxidase in the absence of oxygen does not contribute to physiological NO metabolism.

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