Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3

T Shiomi; K Fukushima; N Suzuki; N Nakashima; E Noguchi; T Nishimoto

doi:10.1093/oxfordjournals.jbchem.a022020

Human dis3p, which binds to either GTP- or GDP-Ran, complements Saccharomyces cerevisiae dis3

J Biochem. 1998 May;123(5):883-90. doi: 10.1093/oxfordjournals.jbchem.a022020.

Authors

T Shiomi¹, K Fukushima, N Suzuki, N Nakashima, E Noguchi, T Nishimoto

Affiliation

¹ Department of Molecular Biology, Division of Dental Science, Graduate School of Medical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

PMID: 9562621
DOI: 10.1093/oxfordjournals.jbchem.a022020

Abstract

Saccharomyces cerevisiae Dis3p, which interacts with Ran/Gsp1p, complements Schizosaccharomyces pombe dis3-54. Consistent with the functional conservation of Dis3p in S. cerevisiae and S. pombe, the human ORF (accession number: R27667) was found to be highly homologous to yeast Dis3p. Based on its nucleotide sequence, we cloned a full-sized human DIS3 cDNA. The cloned human cDNA partly but significantly restored the temperature-sensitivity of S. cerevisiae dis3. Thus, Dis3p was found to be structurally and functionally conserved from yeast to mammals. Consistent with the report that S. cerevisiae Dis3p is identical to Rrp44p, which comprises the exosome involved in ribosomal RNA processing, S. cerevisiae Dis3p was found to be localized in the nucleolus. Similar to S. cerevisiae Dis3p, human Dis3p enhanced RCC1-stimulated nucleotide release from Ran, in a dose-dependent manner, and bound to GTP- or GDP-Ran.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Cell Nucleus / metabolism
Cloning, Molecular
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
Exoribonucleases
Exosome Multienzyme Ribonuclease Complex
Fungal Proteins / genetics
Fungal Proteins / metabolism*
Fungal Proteins / physiology
GTP-Binding Proteins / metabolism*
Genetic Vectors
Guanosine Diphosphate / metabolism*
Guanosine Triphosphate / metabolism*
Humans
Molecular Sequence Data
Nuclear Proteins / metabolism*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae / ultrastructure
Saccharomyces cerevisiae Proteins
Schizosaccharomyces pombe Proteins
Sequence Alignment
Temperature
ran GTP-Binding Protein

Substances

DNA, Complementary
Fungal Proteins
Nuclear Proteins
Saccharomyces cerevisiae Proteins
Schizosaccharomyces pombe Proteins
Guanosine Diphosphate
Guanosine Triphosphate
Exoribonucleases
Exosome Multienzyme Ribonuclease Complex
DIS3 protein, S cerevisiae
dis3 protein, S pombe
GTP-Binding Proteins
ran GTP-Binding Protein

Associated data

GENBANK/AB001743