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Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):4837-40.

Highly resolved chemical imaging of living cells by using synchrotron infrared microspectrometry.

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1
Commissariat à l'Energie Atomique-Institut National des Sciences et Techniques Nucléaires, F91191 Gif Sur Yvette Cedex, France.

Abstract

Using synchrotron radiation as an ultra-bright infrared source, we have been able to map the distributions of functional groups such as proteins, lipids, and nucleic acids inside a single living cell with a spatial resolution of a few microns. In particular, we have mapped the changes in the lipid and protein distributions in both the final stages of cell division and also during necrosis.

PMID:
9560189
PMCID:
PMC20174
[Indexed for MEDLINE]
Free PMC Article
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