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J Bacteriol. 1998 Apr;180(8):2063-71.

Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli.

Author information

1
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA. kenan.murphy@ummed.edu

Abstract

Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate is at least 70-fold higher than that exhibited by a recBC sbcBC or recD strain. The value of the system is highlighted by gene replacement with a PCR-generated DNA fragment. The deltarecBCD::Plac-red kan replacement allele can be P1 transduced to other E. coli strains, making the hyper-Rec phenotype easily transferable.

PMID:
9555887
PMCID:
PMC107131
[Indexed for MEDLINE]
Free PMC Article

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