Implications for cAMP-dependent protein kinase in the maintenance of the interphase state

Prog Cell Cycle Res. 1995:1:241-53. doi: 10.1007/978-1-4615-1809-9_19.

Abstract

The cAMP dependent protein kinase (A-kinase) is one of the first and best studied kinases in mammalian cells. There is extensive evidence that A-kinase activity acts antagonistically toward mitotic entry both in oocyte and somatic cells. Firstly, A-kinase seems to directly compromise the activation process of the cdc2 cyclin B mitotic kinase. Secondly, as shown by specific in vivo inhibition of A-kinase using microinjection of a stable form of its inhibitor peptide PKI, A-kinase modulates several key interphase cellular processes including cytoskeletal dynamics, transcription, chromatin structure and nuclear localization. We discuss the potential mechanisms involved in the down regulation of A-kinase activity at the interphase/mitosis transition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • CDC2 Protein Kinase / metabolism
  • Cell Nucleus / enzymology
  • Cells, Cultured
  • Chromatin / physiology
  • Cyclic AMP / metabolism
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Down-Regulation
  • Fibroblasts
  • Gene Expression Regulation
  • Intermediate Filaments / physiology
  • Interphase*
  • Mammals
  • Mitosis
  • Transcription, Genetic

Substances

  • Chromatin
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • CDC2 Protein Kinase