Abstract
Three helicase structures have been determined recently: that of the DNA helicase PcrA, that of the hepatitis C virus RNA helicase, and that of the Escherichia coli DNA helicase Rep. PcrA and Rep belong to the same super-family of helicases (SF1) and are structurally very similar. In contrast, the HCV helicase belongs to a different super-family of helicases, SF2, and shows little sequence homology with the PcrA/Rep helicases. Yet, the HCV helicase is structurally similar to Rep/PcrA, suggesting preservation of structural scaffolds and relationships between helicase motifs across these two super-families. The comparison study presented here also reveals the existence of a new helicase motif in the SF1 family of helicases.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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DNA Helicases / ultrastructure*
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DNA-Binding Proteins*
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Escherichia coli / enzymology
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Hepacivirus / enzymology*
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Models, Molecular
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Molecular Sequence Data
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Protein Structure, Secondary
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Protein Structure, Tertiary
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RNA Helicases
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RNA Nucleotidyltransferases / ultrastructure*
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Sequence Alignment
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Trans-Activators / ultrastructure*
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Viral Nonstructural Proteins / ultrastructure*
Substances
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DNA-Binding Proteins
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NS3 protein, hepatitis C virus
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Trans-Activators
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Viral Nonstructural Proteins
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replication initiator protein
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RNA Nucleotidyltransferases
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DNA Helicases
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RNA Helicases
Associated data
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PIR/A29457
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PIR/A298900
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PIR/A45068
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PIR/JS0014
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PIR/S10660
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PIR/S39923
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SWISSPROT/P03586
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SWISSPROT/P08411
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SWISSPROT/P09980
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SWISSPROT/P15038
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SWISSPROT/P17769
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SWISSPROT/P28726
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SWISSPROT/P28931
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SWISSPROT/P45157