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Vet Immunol Immunopathol. 1997 Dec 12;60(1-2):1-13.

Organization of the equine immunoglobulin constant heavy chain genes. I. c epsilon and c alpha genes.

Author information

1
Immunology Unit, Hannover School of Veterinary Medicine, Germany. bwagner@immunologie.tiho-hannover.de

Abstract

We provide a restriction map of the equine c epsilon and c alpha genes as a molecular basis for isotype classification. Human and murine DNA probes were used for identification of homologous equine DNA sequences and for isolation of the equine c epsilon and c alpha genes from a genomic DNA library. A detailed map of the equine 5'-s epsilon/c epsilon-s alpha/c alpha-3' gene region was obtained. Equine c epsilon and c alpha DNA probes were prepared and used for restriction analysis of immunoglobulin heavy chain gene loci from different horses. This analysis indicated the presence of only one equine c epsilon and one c alpha gene in the haploid equine genome. In addition, for the equine c alpha gene, four haplotypes were identified according to BamHI restriction fragment length polymorphism (RFLP) of genomic DNA. The relative location of the c epsilon and c alpha genes 3' of the equine c mu and c gamma genes was determined by restriction analysis of equi-murine heterohybridomas.

PMID:
9533263
DOI:
10.1016/s0165-2427(97)00088-3
[Indexed for MEDLINE]

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