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Int J Infect Dis. 1998 Jan-Mar;2(3):159-63.

Outbreak of Salmonella enteritidis gastroenteritis: investigation by pulsed-field gel electrophoresis.

Author information

1
Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, Malaysia.

Abstract

OBJECTIVE:

Pulsed-field gel electrophoresis (PFGE) was used to investigate an outbreak of gastroenteritis caused by Salmonella enteritidis. The outbreak occurred among university undergraduates who consumed contaminated food.

METHOD:

Molecular typing was done by analyzing DNA band patterns of isolates of S. enteritidis after digestion of chromosomal DNA with infrequently-cutting restriction endonucleases XbaI, AvrII, and SpeI and separation of DNA fragments using PFGE.

RESULTS:

Twenty-nine outbreak isolates of S. enteritidis had identical or highly similar PFGE patterns, whereas different PFGE patterns were observed among three epidemiologically unrelated isolates obtained during the same period.

CONCLUSION:

The data obtained confirm the value of PFGE in epidemiologic investigations of outbreaks caused by S. enteritidis.

PMID:
9531664
[Indexed for MEDLINE]
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