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Biotechniques. 1998 Mar;24(3):458-61.

Simultaneous detection of two GFP spectral mutants during in vivo confocal microscopy of migrating Dictyostelium cells.

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1
Universit√§t M√ľnchen, Germany.

Abstract

A method is described that allows simultaneous measurement of two spectrally distinguishable green fluorescent protein (GFP) mutants with a confocal microscope. In contrast to previously described methods, neither UV excitation nor repetition of scans is required. Therefore the method is well-suited to the long-time observation of living cells in three-dimensional microscopy and time series recording, as demonstrated with GFP-expressing Dictyostelium discoideum cells.

PMID:
9526658
DOI:
10.2144/98243st06
[Indexed for MEDLINE]
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