Analysis of anti-sperm sera for T/t locus-specific antibody

J Reprod Immunol. 1979 Jan-Feb;1(1):11-21. doi: 10.1016/0165-0378(79)90025-1.

Abstract

An objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. Each of these antisera was raised against spermatozoa from mice carrying T/t regin mutations. Whilst all of the antisera had considerable activity for mouse spermatozoa, none of them was found to contain antibodies specific for T/t region gene products. A detailed analysis of a single serum revealed that its activity is for antigens which are non-polymorphic, sperm-specific and species-specific. The failure in this study to detect antibodies specific for T/t region gene products is discussed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Absorption
  • Animals
  • Antibodies, Anti-Idiotypic
  • Antibody Specificity*
  • Binding Sites
  • Chromosome Mapping*
  • Epitopes
  • Immune Sera*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mutation
  • Rabbits
  • Radioimmunoassay
  • Rats
  • Spermatozoa / immunology*

Substances

  • Antibodies, Anti-Idiotypic
  • Epitopes
  • Immune Sera