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J Chromatogr B Biomed Sci Appl. 1998 Feb 13;705(2):183-92.

Improved high-performance liquid chromatographic separation of peptidoglycan isolated from various Staphylococcus aureus strains for mass spectrometric characterization.

Author information

1
Robert Koch Institute, Berlin, Germany.

Abstract

Reversed-phase high-performance liquid chromatography (RP-HPLC) of muropeptides, obtained by muramidase digestion of peptidoglycan in combination with amino acid analysis and plasma desorption time-of-flight mass spectrometry is today by far the best tool to analyze the fine structure of the peptidoglycans. Here we report further improvements of the RP-HPLC separation of muropeptides for analyzing the peptidoglycans of various methicillin-resistant strains of Staphylococcus aureus, with emphasis on a more detailed characterization of the interpeptide bridge of the peptidoglycans of this species.

PMID:
9521554
DOI:
10.1016/s0378-4347(97)00506-9
[Indexed for MEDLINE]

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