Using a new technique for scanning electron microscopic preparation, bacterial glycocalyx is seen in its natural, highly hydrated state for the first time. Visual images of the glycocalyx obtained from these preparations are a marked departure from the visual images of glycocalyx obtained previously with conventional scanning electron microscopic analysis. The dominating presence of glycocalyx visualized in its naturally hydrated state gives credence to the role of bacterial glycocalyx as a mechanical barrier to host defenses and antibiotics and supports the role of glycocalyx as a significant factor in bacterial virulence.