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J Med Microbiol. 1997 Aug;46(8):675-80.

Protective activity of Borrelia duttonii-specific immunoglobulin subclasses in mice.

Author information

1
Department of Microbiology, Yamaguchi University School of Medicine, Ube, Japan.

Abstract

To analyse the immune response of mice to Borrelia duttonii infection, BALB/c mice were inoculated intraperitoneally with B. duttonii strain 406K, and the titres of B. duttonii-specific immunoglobulins - IgM, IgG1, IgG2a, IgG2b and IgG3 - were determined by ELISA. IgM antibodies appeared first, followed by IgG2a and IgG3, and then IgG1 and IgG2b. The protective activity of individual classes and subclasses of B. duttonii-specific immunoglobulins was then determined by passive immunisation of BALB/c mice with immunoglobulin preparations purified by affinity chromatography. The mice were then challenged by intraperitoneal inoculation of B. duttonii. The study demonstrated that B. duttonii-specific IgM and IgG3 protected against the development of spirochaetaemia and death after borrelial infection, whereas B. duttonii-specific IgG1, IgG2a and IgG2b had low protective activities.

PMID:
9511816
DOI:
10.1099/00222615-46-8-675
[Indexed for MEDLINE]

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