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J Antimicrob Chemother. 1998 Jan;41(1):123-5.

PCR single strand conformational polymorphism can be used to detect the gene encoding SHV-7 extended-spectrum beta-lactamase and to identify different SHV genes within the same strain.

Author information

1
Department of Microbiology, University of Leeds, UK.

Abstract

The PCR single strand conformational polymorphism (PCR-SSCP) technique described to identify mutants of the SHV beta-lactamases was extended to identify an SHV-7 type beta-lactamase. This was found in a strain of Klebsiella pneumoniae, the first recorded isolate in the UK to produce this type of enzyme. We also demonstrate that PCR-SSCP can be used to identify more than one SHV beta-lactamase gene in a single strain.

PMID:
9511048
DOI:
10.1093/jac/41.1.123
[Indexed for MEDLINE]

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