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Mol Pathol. 1997 Oct;50(5):254-6.

A differential PCR assay for the detection of c-erbB 2 amplification used in a prospective study of breast cancer.

Author information

1
Molecular Genetics Department, Norfolk and Norwich Hospital, UK.

Abstract

AIMS:

To establish a robust differential polymerase chain reaction (PCR) assay for the detection of c-erbB 2 amplification in breast cancer that can be used in a routine pathology laboratory. Once established, the assay was used in a prospective study of breast tumours to investigate the relation between c-erbB 2 amplification and both recognised prognostic features and short term clinical outcome.

METHODS:

The differential PCR was used for the co-amplification of c-erbB 2 and a reference gene from 48 tumour DNA samples and control DNA samples. The ratio of the two genes was determined by image analysis of the PCR products electrophoresed on a highly resolving agarose gel.

RESULTS:

The differential PCR assay was shown to be accurate and reproducible using the conditions outlined. Twenty six per cent of the breast cancer patients were shown to have c-erbB 2 amplification in their tumour biopsies. Twenty eight per cent of the patients died of their disease or had disease recurrence during the follow up period and 73% of these patients had amplification of c-erbB 2.

CONCLUSIONS:

A significant association was found between c-erbB 2 amplification and early disease recurrence. This assay could be used to provide a marker for poor prognosis in breast cancer.

PMID:
9497915
PMCID:
PMC379641
[Indexed for MEDLINE]
Free PMC Article

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