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Biochem J. 1976 Jun 1;155(3):705-7.

Removal of adsorbed elastase from partially digested elastin.

Abstract

Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.

PMID:
949330
PMCID:
PMC1172896
DOI:
10.1042/bj1550705
[Indexed for MEDLINE]
Free PMC Article

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