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FEBS Lett. 1998 Jan 30;422(2):221-4.

Synergistic interaction of the cellulosome integrating protein (CipA) from Clostridium thermocellum with a cellulosomal endoglucanase.

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1
Laboratory of Molecular Enzymology, The Babraham Institute, Cambridge, UK.

Abstract

Activity of a cellulosomal endoglucanase (endoglucanase E; EGE) from Clostridium thermocellum against two crystalline forms of cellulose was enhanced by combination with the cellulosome integrating protein (CipA), but CipA did not enhance EGE activity against amorphous cellulose, even though it was able to bind to it. Similarly, CipA added in trans to genetically truncated EGE that was unable to combine with it nevertheless enhanced EGE activity against crystalline cellulose. These results indicate that the CipA cellulose binding domain does not mediate an increase in activity solely by bringing the catalytic subunits of the cellulosome complex into intimate contact with the substrate.

PMID:
9490011
DOI:
10.1016/s0014-5793(97)01590-1
[Indexed for MEDLINE]
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