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Lett Appl Microbiol. 1998 Jan;26(1):9-11.

A mimic as internal standard to monitor PCR analysis of food-borne pathogens.

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National Food Administration, Uppsala, Sweden.


This report describes a method for the construction of a mimic molecule for monitoring PCR. A blue cheese homogenate spiked with Yersinia enterocolitica was used to demonstrate how false-negative PCR results may be avoided using the mimic. Further, the possibility of estimating bacterial numbers using the mimic was illustrated by varying the target concentration.

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