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Biochem Biophys Res Commun. 1998 Feb 13;243(2):474-9.

Cloning and expression of the human galanin receptor GalR2.

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1
Institute for Metabolic Disorders, Bayer Corporation, West Haven, Connecticut 06516, USA. btb@wh.bayer.com

Abstract

Galanin is a peptide hormone which modulates a wide variety of physiological processes, including secretion, muscle contraction, cognitive function, the reproductive axis, and feeding. Two galanin receptor subtypes, GalR1 and GalR2, have been cloned; however, for GalR2 only the rat sequence has been reported in the literature. Our cloning of human GalR2 reveals its amino acid sequence to be 85% identical to rat GalR2 and 39% identical to human GalR1. Binding of [125I]galanin to the human GalR2 receptor transiently expressed in COS-7 cells was saturable (Kd = 0.24 nM +/- 0.06 nM) with a receptor density of 383 +/- 66 fmol/mg protein. Human galanin(1-30) bound with high affinity to the human GalR2 receptor, with a Ki value of 0.86 +/- 0.12 nM. With the identification of a second galanin receptor subtype, the specific functions of human galanin receptor subtypes can now begin to be addressed.

PMID:
9480833
DOI:
10.1006/bbrc.1998.8133
[Indexed for MEDLINE]
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