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J Cell Biol. 1998 Feb 23;140(4):961-72.

CAS/Crk coupling serves as a "molecular switch" for induction of cell migration.

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1
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA. klemke@scripps.edu

Abstract

Carcinoma cells selected for their ability to migrate in vitro showed enhanced invasive properties in vivo. Associated with this induction of migration was the anchorage-dependent phosphorylation of p130CAS (Crk-associated substrate), leading to its coupling to the adaptor protein c-CrkII (Crk). In fact, expression of CAS or its adaptor protein partner Crk was sufficient to promote cell migration, and this depended on CAS tyrosine phosphorylation facilitating an SH2-mediated complex with Crk. Cytokine-stimulated cell migration was blocked by CAS lacking the Crk binding site or Crk containing a mutant SH2 domain. This migration response was characterized by CAS/Crk localization to membrane ruffles and blocked by the dominant-negative GTPase, Rac, but not Ras. Thus, CAS/Crk assembly serves as a "molecular switch" for the induction of cell migration and appears to contribute to the invasive property of tumors.

PMID:
9472046
PMCID:
PMC2141747
[Indexed for MEDLINE]
Free PMC Article

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