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Appl Environ Microbiol. 1998 Feb;64(2):795-9.

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA.

Author information

1
School of Pure and Applied Biology, University of Wales College of Cardiff, United Kingdom. marchesi@cf.ac.uk

Erratum in

  • Appl Environ Microbiol 1998 Jun;64(6):2333.

Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

PMID:
9464425
PMCID:
PMC106123
[Indexed for MEDLINE]
Free PMC Article

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