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Hepatology. 1998 Feb;27(2):608-14.

Pancreastatin receptor is coupled to a guanosine triphosphate-binding protein of the G(q/11)alpha family in rat liver membranes.

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Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Unidad de Investigación Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain.


Pancreastatin (PST), a recently discovered regulatory peptide derived from chromogranin A, has been shown to have a glycogenolytic effect in the hepatocyte that is mediated by increasing intracellular calcium. Our previous studies on pancreastatin signaling suggested that PST receptor is coupled to some G proteins in the plasma membrane of the hepatocyte. The nature of this interaction was investigated using antisera against G(q/11)alpha by different approaches. Indirect evidence of a pertussis toxin (PT)-insensitive G protein of the family of G(q/11)alpha was obtained by measuring high-affinity guanosine triphosphatase (GTPase) activity in soluble rat liver membranes. PST increased GTPase activity in a dose-dependent manner. This effect was only slightly inhibited by PT pretreatment of the membranes, whereas anti-G(q/11)alpha antisera blocked most of the PST-stimulated GTPase activity. The selective association of the PST receptor with this G protein was further studied by the coelution in wheat germ agglutinin semipurification of the receptor and by immunoprecipitation of the G protein-PST receptor complexes using G-protein-specific antisera. A G protein of the family of G(q/11)alpha was found to be associated with the semipurified PST receptor. Moreover, anti-G(q/11)alpha antisera immunoprecipitated most PST-binding activity (95%), bringing down most of the specific G protein, whereas anti-G(il,2)alpha and -G(o,i3)alpha failed to immunoprecipitate the PST-binding activity. Finally, the coupling of the PST receptor with the effector phospholipase C was disrupted by blocking with G(q/11)alpha antisera, suggesting that a G protein of the family of G(q/11)alpha is a signal mediator from PST receptors to phospholipase C activation in rat liver membranes.

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