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Tissue Antigens. 1997 Dec;50(6):688-92.

High-resolution HLA-DQB1 typing using the polymerase chain reaction and sequence-specific primers.

Author information

1
Oxford Transplant Centre, Nuffield Department of Surgery, Churchill Hospital, United Kingdom. cmulligh@hgmp.mrc.ac.uk

Abstract

Polymorphism at HLA-DQB1 is known to influence tissue compatibility and disease susceptibility; however, current DQB1 typing methods are unable to distinguish the 32 currently recognized DQB1 alleles. We have developed a 32-reaction PCR-SSP method capable of differentiating all DQB1 alleles that differ in amino acid sequence. This method can resolve all heterozygous combinations of DQB1 alleles, with the exception of several combinations involving alleles not thus far detected in Caucasoid populations.

[Indexed for MEDLINE]

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