Send to

Choose Destination
FEBS Lett. 1997 Dec 22;420(1):39-42.

A series of vectors to construct lacZ fusions for the study of gene expression in Schizosaccharomyces pombe.

Author information

Instituto de Investigaciones Biomédicas C.S.I.C. Unidad de Bioquímica y Genética de Levaduras, Madrid, Spain.


We have constructed a series of plasmids to facilitate the fusion of promoters with or without coding regions of genes of Schizosaccharomyces pombe to the lacZ gene of Escherichia coli. These vectors carry a multiple cloning region in which fission yeast DNA may be inserted in three different reading frames with respect to the coding region of lacZ. The plasmids were constructed with the ura4+ or the his3+ marker of S. pombe. Functionality of the plasmids was tested measuring in parallel the expression of fructose 1,6-bisphosphatase and beta-galactosidase under the control of the fbp1+ promoter in different conditions.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center