The STT3 protein is a component of the yeast oligosaccharyltransferase complex

U Spirig; M Glavas; D Bodmer; G Reiss; P Burda; V Lippuner; S te Heesen; M Aebi

doi:10.1007/s004380050611

The STT3 protein is a component of the yeast oligosaccharyltransferase complex

Mol Gen Genet. 1997 Nov;256(6):628-37. doi: 10.1007/s004380050611.

Authors

U Spirig¹, M Glavas, D Bodmer, G Reiss, P Burda, V Lippuner, S te Heesen, M Aebi

Affiliation

¹ Mikrobiologisches Institut, ETH Zürich, ETH Zentrum, Switzerland.

PMID: 9435788
DOI: 10.1007/s004380050611

Abstract

N-linked protein glycosylation is an essential process in eukaryotic cells. In the central reaction, the oligosaccharyltransferase (OTase) catalyzes the transfer of the oligosaccharide Glc3Man9GlcNac2 from dolicholpyrophosphate onto asparagine residues of nascent polypeptide chains in the lumen of the endoplasmic reticulum. The product of the essential gene STT3 is required for OTase activity in vivo, but is not present in highly purified OTase preparations. Using affinity purification of a tagged Stt3 protein, we now demonstrate that other components of the OTase complex, namely Ost1p, Wbp1p and Swp1p, specifically co-purify with the Stt3 protein. In addition, different conditional stt3 alleles can be suppressed by overexpression of either OST3 and OST4, which encode small components of the OTase complex. These genetic and biochemical data show that the highly conserved Stt3p is a component of the oligosaccharyltransferase complex.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Amino Acid Sequence
Carbohydrate Sequence
Fungal Proteins / genetics
Fungal Proteins / isolation & purification
Fungal Proteins / metabolism*
Gene Deletion
Gene Expression Regulation, Fungal
Glycosylation
Hexosyltransferases*
Membrane Proteins / genetics
Membrane Proteins / isolation & purification
Membrane Proteins / metabolism*
Molecular Sequence Data
Multienzyme Complexes / chemistry*
Multienzyme Complexes / isolation & purification
Oligosaccharides / metabolism*
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins*
Sequence Alignment
Sequence Homology, Amino Acid
Transferases / genetics
Transferases / isolation & purification
Transferases / metabolism*

Substances

Fungal Proteins
Membrane Proteins
Multienzyme Complexes
Oligosaccharides
Saccharomyces cerevisiae Proteins
Transferases
Hexosyltransferases
STT3 protein, S cerevisiae
dolichyl-diphosphooligosaccharide - protein glycotransferase