Send to

Choose Destination
Plant Mol Biol. 1997 Dec;35(6):1053-6.

Isolation and characterisation of tobacco (Nicotiana tabacum) cDNA clones encoding proteins involved in magnesium chelation into protoporphyrin IX.

Author information

Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany.


We have identified cDNA clones encoding the two Mg chelatase subunits CHL I and CHL H from tobacco (Nicotiana tabacum) by screening a cDNA library with homologous cDNA fragments from Arabidopsis thaliana. A full-length Chl I cDNA clone encodes a peptide with 426 amino acids. The entire cDNA sequence encoding 1382 amino acid long CHL H was obtained by extension of a truncated cDNA fragment using the 'rapid amplification of cDNA ends' (RACE) method. Both genes Chl I and Chl H were strongly expressed in young leaves and to a lesser extent in mature leaves. Only traces of both transcripts were found in flowering organs. Southern blot analysis suggests that CHL I is encoded by a single gene and CHL H most likely by several genes.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center