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Virology. 1997 Dec 8;239(1):176-85.

Cleavage of transcriptional activator Oct-1 by poliovirus encoded protease 3Cpro.

Author information

1
Department of Microbiology and Immunology, UCLA School of Medicine 90095-1747, USA.

Abstract

In HeLa cells, RNA polymerase II mediated transcription is severely inhibited by poliovirus infection. Both basal and activated transcription are affected to bring about a complete shutoff of host cell transcription. We demonstrate here that the octamer binding transcription factor, Oct-1, is cleaved in HeLa cells infected with poliovirus. Incubation of Oct-1 with the purified, recombinant 3Cpro results in the generation of the cleaved Oct-1 product seen in virus infected cells. Poliovirus infection leads to the formation of altered Oct-1 DNA complexes that can also be generated by incubation of Oct-1 with purified 3Cpro. We also show that Oct-1 cleaved by 3Cpro loses its ability to inhibit transcriptional activation by the SV40 B enhancer. These results suggest that cleavage of Oct-1 in poliovirus infected cells leads to the loss of its activity.

PMID:
9426457
DOI:
10.1006/viro.1997.8862
[Indexed for MEDLINE]
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