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Biotechniques. 1997 Dec;23(6):1087-92.

Inexpensive handheld device for the construction of high-density nucleic acid arrays.

Author information

1
Dept. of Molecular Biotechnology, University of Washington, Seattle 98195, USA.

Abstract

We report an easy-to-use, 384-pin handheld arraying and replicating device (ARD) for constructing high-density replicas of nucleic acids and E. coli transformants. We have modified an existing 384-pin tool to include a novel guide system to ensure vertical pin movement and enhance reproducibility. An asymmetric rectangular multiplexing frame is designed to increase the array density to 1536 dots on a standard microplate-size nylon membrane and to reduce the time and effort involved in producing array replicas. Our initial studies used the ARD to construct 1536-dot arrays of ovarian cDNA clones. We have hybridized these arrays with 32P-labeled probes, which resulted in distinctive signals for either visual interpretation or semiautomatic spot detection and signal integration.

PMID:
9421641
DOI:
10.2144/97236st06
[Indexed for MEDLINE]

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