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J Soc Gynecol Investig. 1994 Jan-Mar;1(1):65-8.

Whole endometrial fragments form characteristics of in vivo endometriosis in a mesothelial cell co-culture system: an in vitro model for the study of the histogenesis of endometriosis.

Author information

1
Department of Obstetrics and Gynecology, University of Oklahoma Health Science Center, Oklahoma City 73190, USA.

Abstract

OBJECTIVE:

We have previously reported on the effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) on the adhesion of human endometrial stromal cells to peritoneal mesothelial cells in vitro. The relevance of this co-culture system to in vivo endometriosis remains to be established. We contrasted endometrial fragments with endometrial stromal cells to determine their relevance.

METHODS:

Human mesothelial cells were isolated from peritoneal fluid from four normal women via Ficoll-Paque gradient centrifugation at 400 x g for 30 minutes and grown in M199 medium containing epidermal growth factor (10 ng/mL) and 10% fetal calf serum. The homogeneous cells were cultured on collagen-coated plates until a monolayer formed. Endometrial stromal cells or whole endometrial fragments, isolated from endometrial tissue of four normal women, were put on the mesothelial monolayer and cultured at 37 degrees C for 24 days. After fixation, the samples were incubated with monoclonal antibody to epithelial membrane antigen, cytokeratin, and vimentin, and processed with standard immunohistochemical techniques.

RESULTS:

Observation under phase contrast microscopy revealed that, in this co-culture system, whole endometrial fragments demonstrated characteristic morphology of in vivo endometriosis, whereas isolated endometrial stromal cells did not.

CONCLUSION:

Whole endometrial fragments, but not isolated endometrial stromal cells, form morphologically characteristic structures similar to in vivo endometriosis in this co-culture system. It is hoped that this system might be useful for studying certain aspects of the histogenesis of endometriosis.

PMID:
9419749
DOI:
10.1177/107155769400100113
[Indexed for MEDLINE]

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