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FEBS Lett. 1997 Nov 17;417(3):287-91.

Expression cloning and characterization of a renal organic anion transporter from winter flounder.

Author information

1
Abteilung Vegetative Physiologie, Zentrum Physiologie und Pathophysiologie, Georg-August-Universität Göttingen, Germany. nwolff@veg-physiol.med.uni-goettingen.de

Abstract

The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder (Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a Km for PAH of about 20 microM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.

PMID:
9409735
DOI:
10.1016/s0014-5793(97)01304-5
[Indexed for MEDLINE]
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