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Mol Biotechnol. 1997 Oct;8(2):105-22.

Degradative covalent reactions important to protein stability.

Author information

1
Merck Research Laboratories, West Point, PA 19486, USA.

Abstract

Commonly observed chemical modifications that occur in proteins during their in vitro purification, storage, and handling are discussed. Covalent modifications described include deamidation and isoaspartate formation, cleavage of peptide bonds at aspartic acid residues, cystine destruction and thiol-disulfide interchange, oxidation of cysteine and methionine residues, and the glycation and carbamylation of amino groups.

PMID:
9406181
DOI:
10.1007/BF02752255
[Indexed for MEDLINE]

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