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Methods. 1997 Oct;13(2):103-11.

Targeting the secretory pathway of Toxoplasma gondii.

Author information

1
Section of Infectious Diseases, LCI 808, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut, 06520-8022, USA.

Abstract

Little is known about the extent of conservation in the organization of the secretory pathway in organisms as different as prokaryotes, eukaryotes, and humans. The protozoan parasite Toxoplasma gondii allows easy genetic manipulations, and numerous vectors for selection of transgenic parasites have been developed. One approach to study the molecular mechanism of protein sorting and trafficking is the expression of foreign proteins. Here we describe the design and application of a vector that targets proteins to the secretory pathway of T. gondii and yields high-level expression of Escherichia coli reporter proteins. The general strategies and potential problems in expressing foreign proteins in T. gondii are discussed.

PMID:
9405194
DOI:
10.1006/meth.1997.0503
[Indexed for MEDLINE]

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